Earlier in oogenesis, we identified that the same bouquet centrosome MTOC machinery from meiosis (described above), functions as the symmetry-breaking machinery that initiates Bb formation, by localizing Bb components around the centrosome (Elkouby et al., 2016, Plos Biology; highlighted by Schubert 2017, Biology of Reproduction; rev. Elkouby, 2017, Int. J. Dev. Biol; Mytlis et al., 2023, Curr. Opin. In Cell Biol.).
Our ongoing functional studies, demonstrate a mechanistic coupling between meiotic nuclear telomere dynamics and cytoplasmic polarization. We generated mutants in which telomere clustering is disrupted, that strikingly resulted in loss of polarity and in mis-regulation of the centrosome. Thus, we revealed meiotic telomere-dependent centrosome regulation, likely uncovering a fundamental program in meiosis which is coopted for cell polarity (in-preparation), as a model for differentiation coordination in cells.
Our work highlights the bouquet centrosome MTOC as an important cellular organizer. While centrosome regulation in mitosis is relatively well understood, the mechanistic control over critical cell type specific centrosome dynamics and functions in developmental contexts is unclear.
We are now working to decipher the dynamics and regulatory mechanisms of the bouquet MTOC, by various biochemical and morphological factors, which will revisit our understanding of centrosome regulation in early oogenesis.
Our work highlights the bouquet centrosome MTOC as an important cellular organizer. While centrosome regulation in mitosis is relatively well understood, the mechanistic control over critical cell type specific centrosome dynamics and functions in developmental contexts is unclear.
We are now working to decipher the dynamics and regulatory mechanisms of the bouquet MTOC, by various biochemical and morphological factors, which will revisit our understanding of centrosome regulation in early oogenesis.
